Abnormal Macrophage Polarization in Patients with Myelodysplastic Syndrome

Abstract

Background. This study is aimed at assessing the subsets of bone marrow macrophages in patients with myelodysplastic syndrome (MDS) and exploring the role of macrophages in the pathogenesis of MDS. Methods. Thirty-eight newly diagnosed MDS patients were enrolled in the Department of Hematology of General Hospital of Tianjin Medical University from June 2015 to June 2016. Bone marrow monocytes and macrophage subsets (M1/M2) were detected in patients with MDS and normal controls by flow cytometry. M1 macrophages were cultured in vitro, and the expression of IL-1β and TNF-α mRNA was measured using real-time polymerase chain reaction. Results. Compared with the normal control group, the proportion of bone marrow monocytes was higher (2.11±0.93% vs. 3.66±3.38%), and the mean fluorescence intensity of surface molecule CD14 was lower in the higher-risk (HR) MDS group (639.05±359.78 vs. 458.26±306.72, p<0.05). The ratio of M2 macrophages to monocytes was higher in patients with HR-MDS (1.82±2.47% vs. 3.93±3.81%, p<0.05). The ratio of M1 to M2 macrophages was lower in the HR-MDS group (3.50±3.22 vs. 1.80±0.88, p<0.05). The expression of IL-1β and TNF-α mRNA in M1 macrophages was significantly lower in the MDS group (p<0.05). Conclusions. Patients with MDS had abnormal macrophage polarization, which may be involved in the alteration of bone marrow microenvironments.