Analysis of histamin content in tuna fish Thunnus sp. with Elisa method at fishing port of Belawan, North Sumatra, Indonesia

Abstract

Tuna migration in Indonesian waters is part of the world tuna migration path because the territory of Indonesia is located on the border crossing between the Indian Ocean and the Pacific Ocean. Histamine is a biogenic amine produced through bacterial decarboxylase from amino acid histidine, and is mostly found in large quantities in fish from the family Scombridae. Histamine production in fish depends on histidine levels in fish, the presence of decarboxylase enzyme-producing bacteria and environmental conditions. The amount of histamine produced by fish is strongly influenced by temperature, time, and storage conditions and species of fish.study This aims to determine the value of Histamine found in Tuna (Thunnus ssp) caught by fishermen. This research was conducted in July 2018 - August 2018. Samples were taken from the catches of fishermen in Belawan randomly, then tested at the Fish Quarantine Center in Medan Fisheries Product Quality and Safety Control I. Histamine test results obtained were around 2.45234 - 13. 31231 mg / L. It is concluded that the tuna from Belawan is still save for consumption.