Effects of boron (Sodium pentaborate), added instead of tris components, on freezing and post-thaw quality of angora buck semen

Abstract

In this research, we aimed to evaluate freezing and in vitro results of post-thawed Angora goat semen parameters using boron (sodium pentaborate, Na2O.5B2O3.10H2O) instead of other components and as an additive in Tris extender. Mature Angora bucks, with proven fertility, accustomed to artificial vagina, and with normospermic values have been used in the study. Research was carried out with 9 different extenders: Boron extenders prepared by adding different amounts of Boron instead of glucose into standard Tris extender (GIB, 1-a and 1-b, 1-c, 1-d), the standard Tris extenders prepared by only addition of different amounts of Boron (BAD; 2-a, 2-b, 2-c, 2-d) and standard Tris extender (Control). Post-thaw spermatological examinations have been done with sperm analyzer (CASA) and results were recorded as follows; the highest total and progresive motility scores between 9 different extenders belonged to 1-c with 63,34 ± 2,32 and 1-d with %38,27± 0,74, highest VCL, VSL, VAP, LIN, STR and WOB average scores were found in 2-d extender (p<0,001). The percentage of lowest dead spermatozoa rate % 42,175± 1,59 was observed with GIB between main groups (p<0,01). Between extenders, lowest dead spermatozoa rates were recorded as % 36,04± 2,58 and % 36,17± 3,66 in 2-b and 1-c (p<0,001). When abnormal spermatozoa rates were examined, lowest abnormal spermatozoon rate belonged to 2-c extender with 21,29± 0,97 (p<0,001) and abnormal acrosome rates were obtained from %6,57± 1,29 1-a extender with (p<0,01). In conclusion, some positive effects of sodium pentaborate on post-thaw spermatological parameters of Angora buck semen have been demonstrated.