Impedance-based detection of DNA sequences using a silicon transducer with PNA as the probe layer.

63Citations
Citations of this article
84Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Electrochemical impedance measurements were used for the detection of single-strand DNA sequences using a peptide nucleic acid (PNA) probe layer immobilized onto Si/SiO2 chips. An epoxysilane layer is first immobilized onto the Si/SiO2 surface. The immobilization procedure consists of an epoxide/amine coupling reaction between the amino group of the PNA linker and the epoxide group of the silane. A 20-nucleotide sequence of PNA was used. Impedance measurements allow for the detection of the changes in charge distribution at the oxide/solution interface following modifications to the oxide surface. Due to these modifications, there are significant shifts in the semiconductor's flat-band potential after immobilization and hybridization. The results obtained using this direct and rapid approach are supported by fluorescence measurements according to classical methods for the detection of nucleic acid sequences.

Cited by Powered by Scopus

Impedance spectroscopy: Over 35 years of electrochemical sensor optimization

242Citations
N/AReaders
Get full text

Detection of microRNAs using target-guided formation of conducting polymer nanowires in nanogaps

198Citations
N/AReaders
Get full text

Diazonium-protein adducts for graphite electrode microarrays modification: Direct and addressed electrochemical immobilization

164Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Macanovic, A., Marquette, C., Polychronakos, C., & Lawrence, M. F. (2004). Impedance-based detection of DNA sequences using a silicon transducer with PNA as the probe layer. Nucleic Acids Research, 32(2). https://doi.org/10.1093/nar/gnh003

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 29

44%

Researcher 20

30%

Professor / Associate Prof. 15

23%

Lecturer / Post doc 2

3%

Readers' Discipline

Tooltip

Chemistry 20

31%

Engineering 20

31%

Agricultural and Biological Sciences 18

28%

Physics and Astronomy 6

9%

Save time finding and organizing research with Mendeley

Sign up for free