Development of an interspecific hybrid (Triploid) by crossing gossypium hirsutum and G. arboreum

Abstract

The 2 species G. hirsutum and G. arboreum were crossed and triploid hybrid (AAD) was developed as a first step of crossing. The triploid is sterile and its hybridity was confirmed with morphological traits and DNA based SSR markers. The chromosome analysis at metaphase-I exhibited 13AA bivalents and 13D univalents at most of the PMC's studied which also confirmed its triploid nature. The mean chromosome pairing association was recorded as 12.90% (univalent), 10.45% (bivalent), 0.45% (trivalent), and 0.48% (tetravalent). The formation of multivalents depicted genome association between 2 species. The further utility of synthesized triploid with respect to the development of CLCuV resistant introgression genotypes is discussed. © 2011 The Japan Mendel Society.