Isolation, structure elucidation, and antibiofilm activity of α-viniferin from Hopea ferrea bark extract

Abstract

Hopea ferrea, a species within the Dipterocarpaceae family, has long been recognized for its traditional medicinal applications. The aim of this study is to isolate and elucidate the structure of α-viniferin from the bark of H. ferrea and to evaluate its antibiofilm activity against Streptococcus mutans. The isolation process was conducted using Medium Pressure Liquid Chromatography (MPLC) and High-Performance Liquid Chromatography (HPLC), resulting in the successful purification of α-viniferin. Structural elucidation was confirmed in Nuclear Magnetic Resonance (NMR) and comparison with literature data. The biofilm inhibitory potential of α-viniferin was evaluated against S. mutans, a major contributor to dental caries. Our findings demonstrated that α-viniferin inhibits biofilm formation in S. mutans, highlighting its potential for development as a natural agent in oral healthcare applications.