Identification of phenolic acid from ethanol extract leaves binahong (Anredera cordifolia (ten) stennis) and antioxidant activity test

Abstract

Binahong (Anredera cordifolia (Ten.) Stennis) is a plant which comes from Basellaceae family. Also, this plant is also known to have biological activity due to the presence of the bioactive compound. Phenolic acids are bioactive compound and widely used as antioxidants. Identification and testing of antioxidant activity using ethanol extract have been done in this research. Phenolic acids were isolated without hydrolysis (TH fraction), and by acid hydrolysis (fraction HA), and alkaline hydrolysis (HB fraction). Based on the identification using TLC with co-chromatography, UV-Vis and FTIR spectrophotometer, the ethanolic extract of binahong leaves was allegedly suspected contain p-coumaric acid. Based on the results of quantitative analysis using TLC Scanner, it can be seen that the p-coumaric acid content in fractions TH, HA, and HB, 11.4220%; 2.8642%; and 23.3563%, respectively. The result of the antioxidant activity test on ethanol extract was 866.89831 mg/L, while (IC50) value on isolate B was 1263.3333 mg/L.