A Novel PCR-RFLP Genotyping of Flavobacterium psychrophilum Targeting the gyrB Region

Abstract

―A novel PCR-RFLP typing method was developed using PCR primers specific for the B subunit of DNA gyrase gene (gyrB) of Flavobacterium psychrophilum and restriction endonuclease Bsp119I. With this PCR-RFLP, 300 isolates of F. psychrophilum were classified into two genotypes (197 and 103 isolates), and the respective genotypes were correlated with the host fish species from which the isolates were derived. These isolates were further subdivided into 12 genotypes by combining with three previous PCR-RFLPs. It is suggested that this PCR-RFLP typing system can be a useful tool in epizootiological studies on bacterial cold-water disease.