Structure and differential expression of the four members of the Arabidopsis thaliana ferritin gene family

Abstract

Four ferritin genes are found within the complete sequence of the Arabidopsis thaliana genome. All of them are expressed and their corresponding cDNA species have been cloned. The polypeptide sequences deduced from these four genes confirm all the properties of the ferritin subunits described so far, non-exhaustively, from various plant species. All are predicted to be targeted to the plastids, which is consistent with the existence of a putative transit peptide at their N-terminal extremity. They also all possess a conserved extension peptide in the mature subunit. Specific residues for ferroxidase activity and iron nucleation, which are found respectively in H-type or L-type ferritin subunits in animals, are both conserved within each of the four A. thaliana ferritin polypeptides. In addition, the hydrophilic nature of the plant ferritin E-helix is conserved in the four A. thaliana ferritin subunits. Besides this strong structural conservation, the four genes are differentially expressed in response to various environmental signals, and during the course of plant growth and development. AtFer1 and AtFer3 are the two major genes expressed in response to treatment with an iron overload. Under our experimental conditions, AtFer4 is expressed with different kinetics and AtFer2 is not responsive to iron. H2O2 activates the expression of AtFer1 and, to a smaller extent, AtFer3. Abscisic acid promotes the expression of only AtFer2, which is consistent with the observation that this is the only gene of the four to be expressed in seeds, whereas AtFer1, AtFer4 and AtFer3 are expressed in various vegetative organs but not in seeds.