Development of an Enzyme Sensor Utilizing a Novel Fructosyl Amine Oxidase from a Marine Yeast

Abstract

Novel enzyme sensors for fructosyl-valine, a model compound of glycated hemoglobin (HbA1c) were constructed using a newly isolated fructosyl-amine oxidase from a marine yeast Pichia sp. N1-1 strain. Both the mediator-type enzyme sensor using carbon paste electrode and the hydrogen peroxide sensor based enzyme electrode exhibit good linear correlations between 0.2 to 2.7 mM and 0.05 mM to 1.8 mM, respectively.