Antimicrobial activity of lawsonia inermis leaf extract collected from south of algeria touat (Adrar) and tidikelt (in salah)

Abstract

Background and Objectives: The leaves of Lawsonia inermis (henna) are widely used as agent to have antimicrobial properties. It is now considered as a valuable source of natural products with pharmaceutical properties. In this study, an attempt was made to find out the antibacterial potential of Lawsonia inermis leaves from south of Algeria, commonly used in traditional medicine to treat various ailments. Materials and Methods: Cold maceration method was used for the extraction of the natural components from the leaves of the plant grown in two different regions. Different concentrations of ethanolic and aqueous extracts have been prepared for the antimicrobial activity (extract). The antimicrobial activity was performed against the different strains. Results: The results indicated that the yield of crude extracts of henna plant from Tidikelt is more active than Touat’s plant. Its antibacterial potential displayed more antimicrobial activity as compared to Touat extracts. TiEth extracts have shown excellent antimicrobial potency against the majority of strains tested even with moderate concentrations of extracts (1.5 mg mLG1). Aqueous extract does show a low activity against the majority of strains including: Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC25922 and Salmonella enteritidis ATCC 2453 at all concentrations tested. Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231 were sensitive toward of Lawsonia inermis’s leaf extract, while others strains show activity average. Conclusion: The present study helped in identifying the phytochemical constituents present in the extract and its fractions which are responsible for various biological and antibacterial activities.