Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens

Abstract

We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay. Copyright © 2011, American Society for Microbiology. All Rights Reserved.