Journal Article

Bacteriophage cloning and Escherichia coli expression of a human IgM Fab

Human Antibodies (1992) 3(2) 81-85
DOI: 10.3233/HAB-1992-3204
4Citations
Citations of this article
2Readers
Mendeley users who have this article in their library.
Get full text

Abstract

We have combined the molecular biology methods of the polymerase chain reaction and recombinant DNA cloning in bacteriophage fambda to express a human IgM Fab in Escherichia coli using genes derived from an Epstein-Barr vzrus transformed cell line. This method comprises three cDNA amplifications and a single clonzng step, culminating In the stable overexpression of mammalian heterodimeric recombinant protein in a prokaryotic host. © 1992 Butterworth–Heinemann.

Author supplied keywords

Cite

CITATION STYLE

APA

Hay, B. N., Sorge, J. A., & Shopes, B. (1992). Bacteriophage cloning and Escherichia coli expression of a human IgM Fab. Human Antibodies, 3(2), 81–85. https://doi.org/10.3233/HAB-1992-3204

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free