PHYTOCHEMICAL SCREENING, GC-MS CHARACTERIZATION AND IN VITRO ANTIBACTERIAL ACTIVITY OF WHOLE PLANT BLEPHARIS BOERHAVIFOLIA PERS AGAINST MRSA

Abstract

Blepharis boerhavifolia (Acanthaceae) commonly known as kozhimookan has been recognized as a part of traditional medication for the treatment of various diseases. This study was carried out with an objective to investigate phytochem. compounds, antibacterial activity and GC-MS anal. of Blepharis boerhavifolia (Acanthaceae). The crude extracts of Blepharis boerhavifolia revealed the presence of several biol. active phytochems. with the highest quantity of alkaloids, flavonoids and phenols were performed for dichloromethane, chloroform, Et acetate, acetone and methanol extract The antibacterial activity was evaluated against gram pos. organism and gram neg. organism Enterococcus faecalis, Klebsiella pneumonia NCIM 2719, Enterobactorclocea NCIM 2164 and Clin. strain Methicillin resistant staphylococcus aureus (MRSA)by agar diffusion method. The antibacterial activity of ampicillin, ciprofloxacin and methanol extract showed maximum zone of inhibition 10 mm, 30.33 mm and 15 mm against MRSA resp.In addition to that qual. anal. of methanolic crude extract of Blepharis boerhavifolia was analyzed by using GC-MS showed that the presence of a ten different phytochem. compounds The components were identified by compare their retention time and fragmentation patterns with those data stored in the National Institute of Standard and Technol. (NIST) library. The reported chief constituents are Azulene derivatives, Cayophyllene, γ-Neoclovene, β-Humulene, Methanoazulene, Tetradecanoic acid and 13-Tetradecenal characterized. The GC-MS anal. revealed the presence of many bioactive compounds such as flavonoids, terpenoids, phenolics and steroids that can be postulated for antibacterial, antioxidant and anticancer activity.