High-density distributed electrodes network for generation of murine induced pluripotent stem cells

Abstract

High-density electrodes network (HDEN) demonstrates a flexible electroporation procedure for gene transfection in different cell types. In this study, the HDEN device shows its capacity as a great candidate in optimization of efficiency in non-viral induction of murine induced pluripotent stem cells (iPSCs) by demonstrating successful expression of different kinds of transgenes of fluorescent proteins. High and steady expression of iPSCs gene expression has been observed. A reasonable transfection rate and viability in different cell types have been demonstrated as well. It implies that a new alternative of iPSCs generation with a combination of plasmid DNA induction and HDEN may be feasible in the near future.