Cadherin-11 mRNA and protein expression in ovarian tumors of different malignancy: No evidence of oncogenic or tumor-suppressive function

Abstract

Cadherin-11 (CDH11, OB-cadherin) is a mesenchymal cadherin found to be upregulated in various types of tumors and implicated in tumor progression and metastasis. In order to determine the role of CDH11 expression in ovarian tumors, we performed a combined reverse transcription quantitative polymerase chain reaction (RT-qPCR), western blot analysis and immunohistochemical study on a large cohort of benign, borderline and invasive ovarian tumors. The RT-qPCR and western blot analysis demonstrated that the CDH11 expression was high in benign cystadenomas and decreased with increasing malignancy. This may be explained by the different tumor-stroma ratios, since immunohistochemistry revealed strong staining of stromal cells, particularly vascular smooth muscle cells and endothelial cells, but only weak cytoplasmic or nuclear immunoreactivity of cancer cells. Within the group of invasive carcinomas, high CDH11 protein expression, as detected by western blot analysis, was found to be significantly correlated with advanced stage and nodal involvement. However, the recurrence-free and overall survival analyses did not reveal any prognostic or predictive significance. In conclusion, in contrast to other tumor types, CDH11 does not play an important role in ovarian cancer progression. 钙粘着蛋白-11（CDH11，OB-钙粘蛋白）是一种间质钙粘蛋白，发现在各种类型的肿瘤中上调并参与肿瘤进展和转移。为了确定CDH11表达在卵巢肿瘤中的作用，我们进行了组合逆转录定量聚合酶链反应（RT-qPCR），免疫印迹分析和免疫组织化学研究大型队列的良性，边缘和侵袭性卵巢肿瘤。 RT-qPCR和蛋白质印迹分析表明CDH11表达在良性囊腺瘤中高，并随着恶性肿瘤的增加而降低。这可以通过不同的肿瘤 - 基质比例来解释，因为免疫组织化学显示基质细胞，特别是血管平滑肌细胞和内皮细胞的强染色，但是仅癌细胞的弱细胞质或核免疫反应性。在侵袭性癌组内，通过蛋白质印迹分析检测到的高CDH11蛋白表达被发现与晚期和淋巴结受累显着相关。然而，无复发和总体生存分析没有显示任何预后或预测显着性。总之，与其他肿瘤类型相反，CDH11不在卵巢癌进展中发挥重要作用。 高CDH11蛋白表达被发现与晚期和淋巴结受累显着相关，与其他肿瘤类型相反，CDH11不在卵巢癌进展中发挥重要作用