Оptimization of blackberry clonal micropropagation

Abstract

One of the modern methods widely used in the propagation of various garden crops is clonal micropropagation. Research in this direction is ongoing to optimize each stage of reproduction. The paper presents the results of a study of the clonal micropropagation of blackberry varieties Brzezina of Polish selection. For clonal micropropagation, sterile BlackBerry microshoots were used. In order to induce adventitious and axillary shoot formation, we tested various variants of culture medium: hormone-free medium, mediumsa with growth regulators (6-benzylaminopurine, β-indolyl-3-butyric acid, gibberellic acid). It was revealed that blackberry microshoots can take root on medium without growth regulators, but the resulting roots are thin, do not have root hairs. Such microshoots does not adapt to ex vitro conditions. A high reproduction rate (96%) was observed when rooting blackberry microshoots in a culture medium ½ MS + 2.0 μM IBA. Adaptation of regenerated plants to ex vitro conditions is more effective (97% survival rate) on a sand + vermiculite substrate (3: 1).