Pengembangan Populasi Mutan Penanda Aktivasi: I. Transformasi Padi Japonica Tropis Lokal Sulawesi cv. Asemandi dengan bantuan Agrobacterium tumefaciens

Abstract

The rice transformation technology is not only provides valuable methods for the introduction of useful genes into rice plant to improve important agronomic traits, but also helps in studying gene function and regulation based on rice genome sequence information. Knockout of genes by insertional mutagenesis is a straightforward method to identify gene functions. One of the methods to develop rice mutants is through genetic transformation mediated by Agrobacterium using activation tagging by Ac-Ds system. A study was done with an objective to obtain mutant rice of local tropical japonica cv. Asemandi through genetic trans-formation mediated by Agrobacterium tumefaciens. The transformation was conducted using Agrobacterium vector with the strain of Agl-1 containing activation tag construct. The result of experiment showed that it has been obtained 17 independent line (304 plants) transgenic Asemandi containing activation tag construct. These starter lines will be used as materials to develop several generations of stabil rice mutant through selfing.