Induction effect of Nigella sativa combined with bovine bone graft on osteoblast and osteoclast formation in post extraction tooth socket

Abstract

Background:. Poor healing of alveolar bone after traumatic tooth extraction procedure increases the difficulty of treatments, especially in the field of prosthodontic. Therefore, adequate socket preservation of alveolar bone is beneficial. Nigella sativa (NS) and bovine bone graft (BBG) is expected to reduce osteoclast and increase osteoblast as a marker of alveolar bone healing. Purpose: This study was aimed to observe the effect of NS combined with BBG on osteoclast and osteoblast count on post-extraction socket of alveolar bone. Methods: Lower incisors of fifty-six Cavia cobaya were extracted and divided into four groups. First group was control group, second group was given BBG, third group with NS, and fourth group with NS and BBG combination. Cavia cobaya was terminated on the 7th and 14th day for further analysis of alveolar socket bone. Histological examinations of osteoclast & osteoblast were measured with a 400x magnification light microscope. One-way ANOVA and Tukey HSD tests were performed to analyze the data statistically. Results: There was a significant difference in osteoclast and osteoblast count between the groups. Both lowest mean of osteoclast and highest mean of osteoblast was found in the NS-BBG combination group on 7th and 14th day. Conclusion: The combination of NS and BBG can effectively decrease osteoclast and increase osteoblast count on post-extraction socket of the alveolar bone.