HPTLC Studies on the Phenyl Propanoids of Albizia lebbeck Benth

Abstract

The present study was aimed to find out the phenyl propanoids HPTLC profile of the medicinally important plant Albizia lebbeck Benth. HPTLC studies were carried out following Harborne and Wagner et al. method. The Toluene-ethyl acetate (9.3:0.7) was served as mobile phase for phenyl propanoids. Linear ascending development was carried out in 20 cm x 10cm twin trough glass chamber (Camag, Mutenz, Switzerland) saturated with the mobile phase and the chromatoplate development for two times with the same mobile phase to get good resolution of phytochemical contents. The developed plate was sprayed with Vanillin sulphuric acid reagent as spray reagent and dried at 120° C in hot air oven for 10 min. The petroleum ether extracts of A. lebbeck leaves displayed the presence of 13 types of phenyl propanoids with 13 different Rf values ranged from 0.02 to 0.92. The ethyl acetate extract of A. lebbeck leaves illustrated the presence of 14 different types of phenyl propanoids with 14 different Rf values with range from 0.01 to 0.94. The methanolic extract of A. lebbeck leaves demonstrated the presence of 6 different types of phenyl propanoids with 6 different Rf values. Maximum number (14) of phenyl propanoids has been observed in ethyl acetate extracts and petroleum ether followed by ethyl acetate (13) extracts of A. lebbeck leaves. The results of the present study developed novel phytochemical marker to identify the medicinally important plant. Further advanced spectroscopic and chromatographic studies will lead for the structural elucidation and identification of active components present in the leaves of A. lebbeck.