Influences of cefotaxime and carbenicillin on plant regeneration from wheat mature embryos

Abstract

The influences of cefotaxime and carbenicillin on regeneration potential of wheat (Triticum aestivum L.) mature embryos were investigated. Filter-sterilized cefotaxime enhanced regeneration capacity although it did not affect the average number of shoots per explant. The highest regeneration capacity of 55.4 % was obtained on regeneration medium supplemented with 100 mg dm-3 cefotaxime. Filter-sterilized carbenicillin did not stimulate plant regeneration. However, higher concentration (100 mg dm-3) accelerated callus browning and inhibited the following regeneration. Autoclaved antibiotics at all tested concentrations showed detrimental effects on callus morphogenesis and plant regeneration. © 2008 Springer Science+Business Media B.V.