Synthesis of licarin A from isoeugenol catalyzed by Brassica juncea peroxidase and its cytotoxicity on MCF-7 cell line

Abstract

By employing crude peroxidases extracted from sawi hijau (Brassica juncea) as biocatalyst, licarin A was synthesized from isoeugenol. The structure was elucidated and confirmed by mass spectrometer (MS) and nuclear magnetic resonance (NMR) analysis. This compound displayed moderate cytotoxicity effect on MCF-7 breast cancer cell with IC50 = 59.95±1.87 μg/mL, compared to doxorubicin with IC50 value of 0.88±0.34 μg/mL as the positive control. These results are indirectly in line with our previously published in silico tests which indicated that licarin A was not a potent ligand for estrogen receptor alpha (ERα), but either a non-ligand or a moderate ligand for ERα.