Use of subcutaneous abdominal fat biopsy specimen for detailed typing of amyloid fibril protein-AL by amino acid sequence analysis

Abstract

A simple technique for the purification of amyloid fibril proteins from patients with systemic amyloidosis was used on a 45 year old woman. The method is based on the use of a surgical subcutaneous fat tissue biopsy specimen which was used for the characterisation of the amyloid as a κ I AL-protein by amino acid sequence analysis. The method permits the exact typing of amyloid in many patients with systemic amyloidosis, which, until now has been almost exclusively confined to necropsy tissue.