Effect of fluoride on endocytosis and surface marker expression levels of mouse B cells in vitro

Abstract

Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 μg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 μM, 100 μM, 500 μM, 1000 μM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50μM. NaF significantly enhanced CD80 expression at 50 μM and decreased CD86 expression at 500 μM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.