Therapeutic Effect of Stereospermum suavelolens on Diabetic Nephropathy

Abstract

Objective: In Indian traditional medicine, decoctions from different parts of Stereospermum suavelolens are used for the treatment of various human diseases including diabetes mellitus. The present study was aimed to evaluate ethyl acetate fraction of ethanol extract from Stereospermum suavelolens for nephroprotective effects in Streptozotocin (STZ)-induced diabetic rats by sub acute model. Methods: The STZ induced diabetic rats were treated once daily, orally with ethyl acetate fraction of Stereospermum suavelolens for 14 days at the doses of 200 and 400 mg/kg. The serum and urine renal function parameters-creatinine, urea, uric acid, albumin and total proteins were measured on 15 th day. The in vivo antioxidant activity of Stereospermum suavelolens was also evaluated in diabetic rats. Effects of the fraction treatments on the kidney histological profile in STZ nephrotoxic rats were also evaluated. Results: The present study investigation showed that the ethyl acetate fraction of Stereospermum suavelolens significantly (P<0.001) attenuated elevations in the serum levels of creatinine, urea, uric acid and total proteins in diabetic treated rats as compared with diabetic control rats. Significant (P<0.001) increase in Superoxide Dismutase (SOD), reduced Glutathione (GSH) and Catalase (CAT) levels and reduction in Thiobarbituric Acid Reactive Substances (TBARS) levels were also observed in ethyl acetate fraction treated rats kidney. The histopathological study of kidney in drug treated rats shows significant protective effect against STZ oxidative stress. Conclusion: Our study concludes that the ethyl acetate fractions of Stereospermum suavelolens possess potent nephroprotective effect against oxidative stress in STZ induced diabetic rats.