1600 Parallel microchamber microfluidic device for fast sample array preparation using the immiscibility of two liquids

Abstract

We present a 1600 parallel microchamber microfluidic device for fast sample array preparation using the immiscibility of two liquids. The trapping efficiency and size of the arrayed sample in the microchambers of a parallel microfluidic device were analyzed at various flow rates. The trapping efficiency of the sample was also inspected according to the position of the microchamber. Samples were successfully arrayed using the device. The trapping efficiency of the sample was 98.69% at 1 mL/h. The trapping efficiency and diameter of the sample decreased as the flow rate increased. Trapping efficiencies also changed according to the position of the microchambers. As the distance of the microchambers from the inlet increased, the sample trapping efficiency decreased. This tendency occurred more clearly at higher flow rates.