Significance of Raffinose Family Oligosaccharides (RFOs) metabolism in plants

Abstract

Raffinose Family Oligosaccharides (RFOs) are a kind of polysaccharide containing D-galactose, and they widely exist in higher plants. Synthesis of RFOs begins with galactinol synthase (GolS; EC 2.4.1.123) to convert myo-inositol into galactinol. The subsequent formation of raffinose and stachyose are catalyzed by raffinose synthase (RS; EC 2.4.1.82) and stachyose synthase (STS; EC 2.4.1.67) using sucrose and galactinol as substrate, respectively. The hydrolysis of RFOs is finished by α-galactosidase (α-Gal; EC 3.2.1.22) to produce sucrose and galactose. Importance of RFOs metabolism have been summarized, e.g. In RFOs translocating plants, the phloem loading and unloading of RFOs are widely reported in mediating the plant development process. Interference function of RFOs synthesis or hydrolysis enzymes caused growth defect. In addition, the metabolism of RFOs involved in the biotic or abiotic stresses was discussed in this review. Overall, this literature summarizes our current understanding of RFOs metabolism and points out knowledge gaps that need to be filled in future.