Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum

Abstract

The increasing usage of chemicals for plant protection in recent years has become a serious problem. One of the possible solutions is use of beneficial microorganisms instead of synthetic fungicides, which will contribute to the protection of the environment and human health. Since the fungi Apegi/JusfLwus and Fusariumgraminearum are the most important pathogens that cause maize diseases and produce mycotoxins, the potential of Tricboderma hat-if arnem for biocontrol of both phytopathogens was examined in this paper. The aim of this paper was to study the influence of different carbon and nitrogen combinations in the medium for T. harifanum production. T. harifanum was cultivated in Erlenmeyer flasks and the effect of cultivation broth against selected maize pathogens was tested using well diffusion method. The results of this study showed that the combination of different carbon and nitrogen sources in the T. hat-if arnem cultivation medium statistically significantly affects the production of Trichoderma cultivation broth effective on two tested phytopathogens. Dextrose as a carbon source and soybean flour as a nitrogen source proved to be the best combination in the medium for production of T. haif anum cultivation broth effective on A. flames and F gramineaem. Maximal inhibition zone diameters of 31 mm and 56.33 mm were registered in those medium formulations for A. flames and F graminearum, respectively. These researches represent an important step for further research in which a medium of low market value would be selected. This would reduce the price of the production process but also the final product.