Antimicrobial Activity of Denture Adhesives Against Lactobacillus, Streptococcus mutans, Candida albicans

Abstract

Denture Stomatitis is a common issue in denture wearers. It is mainly due to its continued use. Once when the patient finds it uncomfortable discontinue the usage. Prevention of mucositis or stomatitis is very important in the denture wearers. It can be prevented by maintaining proper oral hygiene. But in immunocompromised state it is very difficult to maintain especially in old age patients. Denture adhesives are normally used by the denture wearers to have the denture firmly fixed in the mouth. This can be used to prevent the formation of denture stomatitis in the patients. Two types of materials are used by the patients, gel form and powder form adhesives. This study is done to find out the antimicrobial activity of the denture adhesives against the common organisms associated with Denture stomatitis. The antimicrobial effect of the denture adhesive is tested with 3 organisms, C.albicans, S. mutans and Lactobacillus. The anti microbial activity was tested by agar well diffusion method. The media used are, SDA agar and TSA. Wells were cut with a sterile steel tube. TSA was used for Lactobacilli and S. mutans and SDA was used for C.albicans. On each plate 3 wells were cut and the adhesives were filled in it. The adhesives were mixed in sterile Petri dishes and allowed to expand. Thus it was transferred to the well after coating the agar surface with the organism. Then the plates were incubated at 37 degree C for 12 hrs and examined for the zone of inhibition around the well. The zone was measured with a scale and tabulated. The results were obtained as for powder the zone of inhibition in Lactobacillus was 11 mm, zone of inhibition for powder in C.albicans was 14 mm and the zone of inhibition for powder in S.mutans was 10.5 mm which showed a better anti microbial activity for denture adhesive in powder form whereas for gel form the zone of inhibition was not seen. In this study with the 2 products tests, powder has more antimicrobial activity than gel and found to be active against Lactobacillus, Candida albicans and for Streptococcus mutans.