Carbonic anhydrase II binds to the carboxyl terminus of human band 3, the erythrocyte Cl-/HCO3/- exchanger

Abstract

In this study, we provide evidence that the 33-residue carboxyl-terminal (Ct) region of the human erythrocyte chloride/bicarbonate exchanger, band 3, binds carbonic anhydrase II (CAII). Immunofluorescence showed that tomato lectin-mediated clustering of band 3 in ghost membranes caused a similar clustering of CAII, indicating an in situ association. CAII cosolubilized and coimmunoprecipitated with band 3, suggesting that the two proteins form a complex. Band 3 (K( 1/4 ) = 70 nM) or the membrane domain of band 3 (K( 1/4 ) = 100 nM) bound saturably to immobilized CAII in a solid phase binding assay. The interaction with CAII was specifically blocked by an antibody to the Ct of band 3. Affinity blotting showed that a glutathione S-transferase (GST)- fusion protein (GST-Ct) containing the last 33 residues of human band 3 bound to CAII. The solid phase binding assay showed that binding of GST-Ct to immobilized CAII was saturable (K( 1/4 ) = 20 nM). The binding rate was slow (t( 1/4 ) = 12 h) at physiological ionic strength and pH but was enhanced at low ionic strength or acidic pH. Intact band 3 (K(i) = 15 nM), the membrane domain of band 3 (K(i) = 100 nM), or antibodies to the Ct of band 3 were able to block GST-Ct binding to CAII, confirming the specificity of the interaction. Affinity chromatography showed that CAII bound to immobilized GST-Ct with a 1:1 stoichiometry. This work indicates that CAII, the bicarbonate supplier, is directly coupled to band 3, the chloride/bicarbonate exchanger in red blood cells.