Isolation and characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase-containing plant growth-promoting rhizobacteria from carnation soil and roots

Abstract

Five strains of plant growth-promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity were isolated from carnation soil and roots using ACC as the sole nitrogen source. Based on their growth morphological, microscopic cell properties and 16S rRNA sequence analysis, the results showed that three strains were identified as Enterobacter section and one as Erwinia among four strains from the soil, and one from carnation roots wAS identified as Acinetobacter; there are some differences in ACC deaminase activities among all isolated strains in this study. It is suggested that ACC deaminase-containing PGPR could be a cost-effective, environment-friendly and promising potential strategy to promote plant growth, alleviate biotic and abiotic stresses and ensure sustainable agriculture, especially for ethylene-sensitive flowers production.