Isolation, characterization, and cytotoxic studies of secondary metabolites from the leaves of Bauhinia foveolata Dalzell: An endemic tree from the Western Ghats, India

Abstract

Bauhinia foveolata Dalzell is an endemic tree, native to Southwest India (the Western Ghats). 13-Docosenamide was isolated from ethyl acetate fraction, and quercetin (1), isorhamnetin (2), and odoratin-7-glucoside (3) were isolated from butanol fraction of leaves. A spectroscopic analysis, including mass spectra and Nuclear Magnetic Resonance (NMR), and also comparison with reported data were used to elucidate the chemical structures of isolated compounds. Furthermore, all the isolated compounds were analyzed for 3-(4, 5-dimethylthiazol-2-yl)-5-diphenyltetrazolium bromide -based cytotoxicity studies on human colon cancer cell lines, HT-29 and HCT-15; among all the tested ompounds, quercetin and odoratin-7-glucoside have shown a good cytotoxicity toward the selected cell lines.