Rapidly Diagnosing Grapevine Corky-bark by in Vitro Micrografting

Abstract

Grapevine (Vitis vinifera L.) explant shoots indexed forcorky-bark and rootstocks from healthy LN33 indicator plants were sterilized and maintained in vitro. When infected shoot tips were micrografted onto LN33 shoots, typical corky-bark symptoms appeared in 8 to 12 weeks. We suggest developing this method further to replace the regular, 2-year indexing procedure.