Host genetic background and environment have different effects on the establishment and structure of the adult worker honey bee gut microbiota

Abstract

Many studies have highlighted the importance of gut microbiomes to many aspects of host physiology. Therefore, understanding how factors, such as host genetics and environment, impact the establishment and composition of the gut microbiota can provide insight into host biological functioning. Here, we controlled the microbial inoculation of honey bees across distinct social groups, each composed of a mixture of bees from three distinct genetic backgrounds, and each housed in a distinct cage environment. We determined the relative effects of host genetic background and cage environment on the establishment and composition of the gut microbiota. Using 16S rRNA gene sequencing, we found that host genetic background and cage environment had effects on gut microbiota structure, with each influencing gut microbiota beta diversity. In addition, host genetic background and cage environment each affected the abundance of different individual gut microbes. Together, these results suggest that host genetic background and environment may play distinct roles in shaping the establishment and structure of host gut microbiota.