Picroside II protects myocardium from ischemia/reperfusion–induced injury through inhibition of the inflammatory response

Abstract

The inflammatory response is important in the pathogenesis of myocardial ischemia/reperfusion (I/R) injury. Picroside II,the primary active constituent of Picrorhizae,has been reported to protect the myocardium from I/R–induced injury,however,the exact mechanism underlying these protective effects remains unclear. The aim of the present study was to investigate the mechanism underlying the protective effects of picroside II on I/R–induced myocardial injury. Adult male Sprague–Dawley rats underwent 1 h left coronary artery occlusion followed by 3 h reperfusion. Picroside II was administered (10 mg/kg) via the tail vein 30 min prior to left coronary artery occlusion. The results revealed that pretreat­ment of picroside II could significantly alleviate I/R–induced myocardial injury concomitantly with a decrease in inflamma­tory factor production. In addition,picroside II was also able to decrease high mobility group box 1 (HMGB1) expression,and release and downregulate the expression of the receptor for advanced glycation end products (RAGE),toll–like receptor (TLR)–2 and TLR–4. Furthermore,picroside II was able to inhibit nuclear factor–κB (NF–κB) activation. The results indicated that the protective effect of picroside II on I/R–induced myocardial injury was associated,at least partly,with inhibition of the inflammatory response by suppressing the HMGB1–RAGE/TLR–2/TLR–4–NF–κB signaling pathway.