CircRNA circRNA-102171 promotes papillary thyroid cancer progression through modulating CTNNBIP1-dependent activation of β-catenin pathway 06 Biological Sciences 0601 Biochemistry and Cell Biology

Abstract

Background: As a type of recently discovered noncoding RNA, circular RNAs (circRNAs) exert pivot biological functions in diverse cancers. However, the role of circRNA-102171 in papillary thyroid cancer (PTC) has not been investigated. Our study was focused on the functional investigation toward circRNA-102171 in PTC progression. And we also aimed to reveal its potential molecular mechanism. Methods: The expression pattern of circRNA-102171 was determined using quantitative polymerase chain reaction (qPCR) in PTC samples and cell lines. Cell proliferation was examined utilizing CCK8, colony formation and EdU incorporation assays. Apoptosis was analyzed by Annexin V/PI staining and FACS detection. Cell migration and invasion was measured using Transwell assay. Tumor growth in vivo was determined through a xenograft assay. RNA-pulldown, RNA-IP (RIP) and RNA-EMSA were used to analyze the interaction between circRNA-102171 and CTNNBIP1. Results: CircRNA-102171 expression was upregulated in tumor tissues and cell lines. CircRNA-102171 silencing suppressed PTC cell proliferation, migration and invasion while promoting apoptosis. CircRNA-102171 knockdown inhibited PTC growth in vivo. CircRNA-102171 interacted with CTNNBIP1 to block its interaction with the β-catenin/TCF3/TCF4/LEF1 complex, leading to activation of Wnt/β-catenin pathway. Conclusions: CircRNA-102171 overexpression promotes PTC progression through activating Wnt/β-catenin pathway in a CTNNBIP1-dependent way.