Esmolol cardioplegia: The cellular mechanism of diastolic arrest

Abstract

AimsEsmolol, an ultra-short-acting-blocker, acts as a cardioplegic agent at millimolar concentrations. We investigated the mechanism by which esmolol induces diastolic ventricular arrest.Methods and resultsIn unpaced Langendorff-perfused rat hearts, esmolol (0.03-3 mmol/L) had a profound negative inotropic effect resulting in diastolic arrest at 1 mmol/L and above. This inhibition of contraction was maintained during ventricular pacing. At 3 mmol/L, esmolol also abolished action potential conduction. To determine the cellular mechanism for the negative inotropism, we measured contraction (sarcomere shortening) and the calcium transient (fura-2 fluorescence ratio; Catr) in electrically-stimulated rat ventricular myocytes at 23 and 34°C. The decrease in contraction (by 72 at 23°C, from 0.16 ± 0.01 to 0.04 ± 0.01 m, P < 0.001) was similar to that of isolated hearts and was caused by a large decrease in Catr (from 0.13 ± 0.02 to 0.07 ± 0.02, P < 0.001). There was no additional effect on myofilament Ca 2+ sensitivity. Esmolol's effects on contraction and Catr were not shared or altered by the-blocker, atenolol (1 mmol/L). Sarcoplasmic reticulum inhibition with thapsigargin did not alter the inhibitory effects of esmolol. Whole-cell voltage-clamp experiments revealed that esmolol inhibited the L-type calcium current (ICa,L) and the fast sodium current (INa), with IC50 values of 0.45 ± 0.05 and 0.17 ± 0.025 mmol/L, respectively. ConclusionEsmolol at millimolar concentrations causes diastolic ventricular arrest by two mechanisms: at 1 mmol/L (and below), the pronounced negative inotropic effect is due largely to inhibition of L-type Ca2+ channels; additionally, higher concentrations prevent action potential conduction, probably due to the inhibition of fast Na+ channels. © 2010 The Author.