Antimicrobial effect of Triphala mediated gold nanoparticles and its based indigenous mouthrinse against common oral pathogens

Abstract

The aim of the study was to synthesize gold nanoparticles (AuNP’s) by using triphala plant extract and to evaluate antimicrobial activity of gold nanoparticles and its based mouthwash. This study used a triphala extract for nanoparticle synthesis in combination with gold chloride (AuCl2). The AuNP’s were characterized for shape and size using (Transmission electron microscopy) TEM analyses and UV vis spectrophotometry. The gold nanoparticle-based mouthwash (10ml) was prepared using 600 µL nanoparticles. The antimicrobial activity was assessed using agar gel diffusion method. To determine the antimicrobial activity of AuNp’s, the inhibition zones were measured for: Enterococcus faecalis, Streptococcus mutans, Staphylococcus aureus and Candida Albicans at 25µL, 50µL ,100µL concentration and at 25µL ,50µL, 100 µL concentration for mouthwash. AuNP’s showed excellent antimicrobial activity against C. Albicans, S. mutans as compared to S. aureus, E. faecalis which showed good antimicrobial activity with phenomenal activity at 100 µL concentration. Mouthwash showed significant activity against S. aureus and good activity against, E. faecalis, C. Albicans and S. mutans with highest activity at 100 µL. At concentrations of 25 µL, 50 µL, moderate antimicrobial potential was elicited by AuNP’s and mouthwash. Significant antimicrobial activity was elicited by AuNP’s against commonly present oral microorganisms.