Plant growth-promoting effects of rhizospheric and endophytic bacteria associated with different tomato cultivars and new tomato hybrids

Abstract

Background: Conventional agriculture relies on chemical pesticides and fertilizers, which can degrade ecosystems. A reduction of these harmful practices is required, replacing (or integrating) them with more eco-friendly approaches, such as microbial inoculation. Tomato is an important agricultural product, with a high content of bioactive compounds (folate, ascorbate, polyphenols, and carotenoids). The focus of this research was to investigate the plant growth-promoting (PGP) abilities of bacterial strains isolated from different tomato cultivars, with the aim to develop systems to improve plant health and crop productivity based on microbial inoculation. Methods: A pool of different tomato cultivars already available on the market and new tomato hybrids were selected based on their nutritional quality (high content of biologically active compounds). A total of 23 strains were isolated from tomato roots (11 rhizospheric strains and 12 root endophytes). The cultivable isolates were analyzed for a number of different PGP traits: organic acids (OA), indole acetic acid (IAA), ACC deaminase, and siderophore production. The effects of microbial inoculation on root growth of Arabidopsis thaliana were also evaluated using a Vertical Agar Plate assay. Results: A high percentage of the isolated strains tested positive for the following PGP traits: 73 % were able to produce OA, 89 % IAA, 83 % ACC deaminase, and 87 % siderophores. The most striking result were remarkable increases in the formation of root hairs for most of the inoculated plants. This effect was obvious for all A. thaliana seedlings inoculated with the isolated endophytes, and for the 50 % of the seedlings inoculated with the rhizospheric strains. Conclusions: A better knowledge of the plant growth-promotion activity of these strains can provide an important contribution to increase environmental sustainability in agriculture.