Cyanobacterial picoplankton from the Central Baltic Sea: Cell size classification by image-analyzed fluorescence microscopy

Abstract

Enumeration and measurements of cell size in Baltic picoplanktic cyanobacteria were performed by video recording of autofluorescent images of unitcells with a diameter of <3 μm. Statistical analysis was used in order to assess the contribution of the smallest photosynthetic fraction to the biomass and size distribution in the photic zone of the Baltic proper during summer of 1995. The vertical distribution of picocyanobacteria varied between 6.7 and 1.8 x 105 cells ml-1 from the surface to 20 m depth, with an average value of 5.6 and 3.8 x 105 cells ml-1 in July and August, respectively. Three size classes were distinguished on the basis of morphometric analysis, as well as four types of cell aggregates frequently occurring in the samples. Variations were detected between the 2 months in picocyanobacterial populations present in the mixed layer, mainly concerning decrease in cell number, and increase in dimensions in August. Ecological and taxonomic implications arising from the data are discussed.