Isolation of metallic Klebsiella Pneumoniae HHHF1 potent for AgNPs nanobiosynthesis from iron-rich soil samples

Abstract

Biosynthesis of nanoparticles has the upper hand over physical and chemical methods being cost-effective and eco-friendly. Soil is regarded as the major reservoir of microorganisms. Iron-containing soil samples were screened for metallic bacteria. Metallic K. Pneumoniae HHHF1 potent for AgNPs nanobiosynthesis has been isolated from iron-rich soil samples, identified biochemically and genetically by 16sRNA subunit sequencing and submitted to the NCBI. Biogenic AgNPs synthesized using AgN03 as a substrate at a concentration of (10â mM) and cell-free supernatant was indicated by the color changes in the reaction mix from yellow to brown indicating the presence of AgNPs. XRD analysis revealed AgNPs average size 35.799nm. EDS analysis predicted occurrence and percentage of elemental silver 85.78%. Biosynthesized AgNPs showed antibacterial activity against pathogenic bacteria of both gram-positive and gram-negative bacteria (Proteus mirabilis, Shigella soni, Salmonella typhi, Klebsiella pneumonia, Enterobacter spp., Serratia marcescens, E. Coli, Sphingomonas paucimobilis, Pseudomonas aeroginosa, Kocuria spp, and staphylococcus aureus). Isolation of bacterial strains potent for nanobiosynthesis enhances nanobi-osynthesis qualitatively and quantitatively and gives the advantage to study the nano synthetic pathways involved in those bacteria.