Determination of Benzoic Acid, Sodium Saccharin, And Caffeine By High Performance Liquid Chromatography

Abstract

Concentrations of benzoic acid, sodium saccharin and caffeine in soft drink samples were determined by using Reverse Phase High Performance Liquid Chromatography (HPLC). The optimum analytical conditions of those three samples was methanol-phosphate buffer (1:7) as mobile phases, with pH 4.5 and flow rate 1.0 mL/min, column C18 (150x4.6 mm i.d.) as stationary phases at 400C with UV-Vis Spectrophotometer detection at 220 nm. The concentration level of those compounds observed was not beyond the maximum limit of SNI 01-011101995 for sodium saccharin and benzoic acid and SNI 01-6684-2002 for caffeine. The relative standard deviation based on retention time and peak area, as 0.37% and 0.59% for benzoic acid, 0.16% and 0.21% for sodium saccharin, 0.38% and 0.6% for caffeine. Finally, the recovery for benzoic acid, sodium saccharin and caffeine was 100.82%, 94.92% and 90.32%, respectively. The relative standard deviation and recovery of all samples met AOAC method requirements.