In vitro activity of 2-methoxy-1,4-naphthoquinone and stigmasta-7,22-diene- 3β-ol from Impatiens balsamina L. against multiple antibiotic-resistant Helicobacter pylori

Abstract

Infection with Helicobacter pylori is strongly associated with gastric cancer and gastric adenocarcinoma. WHO classified H. pylori as a group 1 carcinogen in 1994. Impatiens balsamina L. has been used as indigenous medicine in Asia for the treatment of rheumatism, fractures and fingernail inflammation. In this study, we isolated anti-H. pylori compounds from this plant and investigated their anti- and bactericidal activity. Compounds of 2-methoxy-1,4-naphthoquinone (MeONQ) and stigmasta-7,22- diene-3β-ol (spinasterol) were isolated from the pods and roots/stems/leaves of I. balsamina L., respectively. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) for MeONQ were in the ranges of 0.156- 0.625 and 0.313-0.625 μ g mL-1, respectively, and in the ranges of 20-80 μ g mL-1 both of MICs and MBCs for spinasterol against antibiotic (clarithromycin, metronidazole and levofloxacin) resistant H. pylori. Notably, the activity ofMeONQwas equivalent to that of amoxicillin (AMX). The bactericidal H. pylori action ofMeONQ was dose-dependent. Furthermore, the activity ofMeONQ was not influenced by the environmental pH values (4-8) and demonstrated good thermal (121.C for 15 min) stability. MeONQ abounds in the I. balsamina L. pod at the level of 4.39% (w/w db). In conclusion,MeONQ exhibits strong potential to be developed as a candidate agent for the eradication of H. pylori infection. Copyright © 2011 Yuan-Chuen Wang et al.