Effectiveness of media B5 (Gamborg) for multiplication of Sengon ( Paraserianthes falcataria L. Nielsen) in vitro

Abstract

Paraserianthes falcataria (L.) Nielsen, also known as sengon, is one of the most important and adaptable pioneer species. The Sengon plant is one of the quickest-growing tropical plants. The objective of this study was to identify the optimal medium for sengon multiplication with the addition of ZPT, cytokinin, BAP, and kinetin, either singly or in combination. The research design employed was an entirely random design (CRD). Media M2 (B5 + BAP 0.1), which was the medium with the highest number of shoots, was determined to be the optimal medium for sengon plantlets in the study. At 1 MST, the fastest medium for leaf emergence was (B5 + BAP 0.2) media. The poisson test analysis shows that, in comparison to other media, the M2 medium (B5 + BAP 0.1) has a very significant impact on the quantity of leaves. The medium with the highest average height of sengon plantlets was M1 (B5 Control), with 1.73 cm. The highest percentage of dead plantlets on M4 medium was 33%, while on M9 medium it was 17%, due to browning leading to cell death. Only media M4 (BAP 0.2), M5 (Kinetin 0.2), and M9 (BAP (0.2) + Kin (0.2) contained plantlets contaminated by bacteria at a concentration of up to 17%, which inhibited plantlet growth.