Influence of NaFeEDTA fortification in soy milk on hemoglobin levels in male Sprague-Dawley rats (Rattus norvegicus L.)

Abstract

In this study, we investigated the influence of the fortification of soy milk with NaFeEDTA on blood hemoglobin levels in male Sprague Dawley rats (Rattus norvegicus L.). Using a completely randomized design, 25 rats were divided into five groups. A normal control group was administered standard feeding without the addition of soy milk and fortificant, whereas a treatment control group was administered soy milk without fortificant; three treatment groups were administered soy milk fortified with the following doses of NaFeEDTA: 2.7 mg Fe/kg bw (treatment group 1), 5.4 mg Fe/kg bw (treatment group 2), and 10.8 mg Fe/kg bw (treatment group 3). The five groups were consecutively treated for 21 days. Rat blood hemoglobin measurements were performed using a hematology analyzer. The results of one-way ANOVA and least significant difference tests demonstrated that after the 21-day consecutive treatment, there was a significant influence on the blood hemoglobin levels in all treatment groups when compared with treatment group 1, whereas treatment groups 2 and 3 exerted a significant influence on the blood hemoglobin levels when compared with the treatment control group. The highest increase in the blood hemoglobin levels was detected in treatment group 2, which was a 10.84 % increase from the normal control group and 9.28 % increase from the treatment control group.