The determination of 2:3-diketo- l -gulonic acid

Abstract

Vol. 37 METABOLISM OF PENICILLIUM 39 chlorinated metabolic product, while growth on media devoid of chloride in no way assisted the isolation of the red component. Variation of the KCI concentration showed that its elimination from the medium was quite regular after the initial period when the sugar metabolism was exceptioAally rapid. Increased chloride concentration had no effect on the production of sclerotiorine and only affected the chlorine content of the other products of extraction of the mycelium. It is suggested that the pigment is a metabolic by-product rather than the fundamental end-product of the chloride metabolism. The acti-vating effects of yeast extract was no more than equivalent to the effect of increased KCI concen-tration.