In Vitro Antioxidant, Antihyperglycemic and Antiglycation Properties of Bulbs, Flower Buds and Flowers Extracts of Lilium Species and their Chemometric Profiling

Abstract

This objective of this study was to explore the antioxidant, antihyperglycemic, and antiglycation properties of 13 methanolic extracts from 8 different Lilium species by various in vitro assays and their chemometric profiles to provide the evidencebased information for further exploration. Chemometric profiling was performed using the MetaboAnalyst and GNPS: Global Natural Products Social Molecular Networking platforms to explore and identify the metabolites from Lilium metabolomics profiles. In vitro bioactivity studies showed that flower bud extract of L. lancifolium was the most active extract with the strongest radical scavenging activity against DPPH and SO radicals, reducing activity of ferric ions and highest total phenolic contents among tested extracts. Univariate and multivariate statistical analysis revealed that the chemical variation between the sample classes: parts used (bulb vs flower); bioactivity (active vs inactive) and studied species. 13 bioactive metabolites were putatively identified and some of them were confirmed by comparing the MS/MS spectrums with the standards. Results suggest that metabolite profiling along with the bioactivity study could be useful to explore the chemical compositions, functional and developmental potentials of valuable plant species, and lay the foundation for identification, separation and assessment of their potential medicinal applications and species classification.