Evaluation of Efficacy of Collagen Material Containing Double-Release Gentamicin Sponge in Preventing Postoperative Spinal Infection: Experimental Study (Animal Experiment)

Abstract

Objective: We studied that collagen material containing double-release gentamicin sponge for preventing of experimental postoperative spinal infection animal model. Material and Methods: A total of 28 adult female rats were used, in 4 groups each having 7 rats. Group 1: Only cutaneous-subcutaneous, fascia opened, muscles stripped; Group 2: Laminectomy of 2 levels+sterile normal saline; Group 3: Laminectomy of 2 levels+Staphylococcus aureus [106 colony forming unit (CFU)/10 ult]; Group 4: Laminectomy of 2 levels+S. aureus (106 CFU/10 ult)+collagen material containing double-release gentamicin sponge. With a light microscope, epidural fibrosis, inflammatory cell infiltration and fibroblast density of sections were evaluated and graded. Results: Group 2 had higher epidural fibrosis, fibroblast cell density and inflammatory cell density than those of Group 1. Group 3 had higher epidural fibrosis, fibroblast cell density and inflammatory cell density than those of Group 1. Group 1 had lower epidural fibrosis, fibroblast cell density and inflammatory cell density than those of Group 4. The inflammatory cell density was higher in Group 3 than that of Group 2. The epidural fibrosis and inflammatory cell density were lower in Group 4 than those of Group 2. The Group 4 had lower epidural fibrosis, fibroblast cell density and inflammatory cell density than those of Group 3. Conclusion: We therefore consider that the material used for this study could be used for prophylaxis to prevent epidural fibrosis and infection in spinal surgery.