Metabolite profile and elemental determination of camel follicular fluid by GC–MS and ICP-MS

Abstract

The objective of present study was to determine metabolite profile and inorganic elements of camel follicular fluids (FF) using “gas chromatography–mass spectrometry (GC–MS) and inductively coupled plasma mass spectrometry (ICP-MS),” respectively. Various metabolites were detected in camel FF by the proposed GC–MS technique. The major compounds detected were lactic acid (62.37%), linolenic acid (5.95%), myo-inositol (3.37%), hexadecanoic acid (3.19%), N-ethyl-N-vinylacetamide (3.15%), acetamide (2.89%), tetradecanoic acid (2.64%), and d-xylofuranose (2.25%). The proposed ICP-MS technique was validated in terms of linearity, precision, accuracy, and sensitivity. All quality control validation parameters were found to be satisfactory for the analysis of elements in camel FF. The proposed ICP-MS technique showed the presence of sixteen different elements (out of eighteen standards) in camel FF. Some elements such as Na, K, Ca, and Mg were obtained in higher amounts in camel FF. Overall, the results of this study indicated that the proposed GC–MS and ICP-MS techniques can be successfully applied for metabolite profile and element determination of biological fluids such as FF.