Oxygen concentrations inside the traps of the carnivorous plants Utricularia and Genlisea (Lentibulariaceae)

Abstract

• Background and Aims: Species of Utricularia and Genlisea (Lentibulariaceae) are carnivorous, capturing small prey in traps which are physiologically very active, with abundant quadrifid and bifid glands. Traps of Utricularia have walls composed of two cell layers, and are filled with water. Diverse communities of commensal microorganisms often live inside the traps. Genlisea forms long, hollow subterranean traps of foliar origin, growing in anoxic wet substrate. Knowledge of the O2 concentrations inside Utricularia and Genlisea traps is vital for understanding their physiological functioning and conditions for the life of commensals. To test the hypothesis that prey are killed by anoxia inside the traps, and to measure respiration of traps, [O2] was measured in the fluid in mature traps of these species. • Methods: Oxygen concentration and electrical redox potential were measured using a small Clark-type oxygen sensor and a miniature platinum electrode, respectively, in the fluid of excised and intact traps of six aquatic Utricularia species and in Genlisea hispidula traps. • Key Results: Steady-state [O2] in the traps of both genera always approached zero (median 0.0-4.7 μm). The [O2] decreased after electrodes were inserted into Utricularia traps at a rate which ranged from 0.09 to 1.23 mm h-1 and was lower in traps of irradiated and intact shoots with higher [O2] in shoot tissues. Redox potential ranged from -24 to -105 mV in the traps, confirming the very small or zero [O2]. • Conclusions: Very small or zero [O2], effectively anoxia, is demonstrated in Utricularia and Genlisea traps. This is probably below the critical [O2] for prey survival, and causes captured prey to die of suffocation. Internal trap glands and trap commensals are considered to be adapted to facultative anoxia interrupted by limited periods of higher [O 2] after firings. © The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.